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primary antibodies against mouse b7-2 (cd86)  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology primary antibodies against mouse b7-2 (cd86)
    Effects of moderate hyperglycemia on the expression of TNFα , Cxcl10 , and macrophage markers in the liver. (A) TNFα expression. (B) Cxcl10 expression. (C–F) macrophage markers. Every gene expression intensity was normalized to that of GAPDH . Bars are mean ± SEM, n=3 ( TNFα ), n=6 ( Cxcl10 , F4/80 , CD68 , <t>CD86</t> , CD206 ). (G) Protein band of CD86 and GAPDH of Wild and GKKO mice liver samples (a) and their intensity profiles analyzed using ImageJ (b). Blue dotted line: Wild, red dotted line: GKKO.
    Primary Antibodies Against Mouse B7 2 (Cd86), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+mouse+b7-2+(cd86)/anti+cd86/pmc10202721-71-42-49
    Average 90 stars, based on 1 article reviews
    primary antibodies against mouse b7-2 (cd86) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Moderate hyperglycemia suppresses melanoma metastasis to liver"

    Article Title: Moderate hyperglycemia suppresses melanoma metastasis to liver

    Journal: Experimental Animals

    doi: 10.1538/expanim.22-0078

    Effects of moderate hyperglycemia on the expression of TNFα , Cxcl10 , and macrophage markers in the liver. (A) TNFα expression. (B) Cxcl10 expression. (C–F) macrophage markers. Every gene expression intensity was normalized to that of GAPDH . Bars are mean ± SEM, n=3 ( TNFα ), n=6 ( Cxcl10 , F4/80 , CD68 , CD86 , CD206 ). (G) Protein band of CD86 and GAPDH of Wild and GKKO mice liver samples (a) and their intensity profiles analyzed using ImageJ (b). Blue dotted line: Wild, red dotted line: GKKO.
    Figure Legend Snippet: Effects of moderate hyperglycemia on the expression of TNFα , Cxcl10 , and macrophage markers in the liver. (A) TNFα expression. (B) Cxcl10 expression. (C–F) macrophage markers. Every gene expression intensity was normalized to that of GAPDH . Bars are mean ± SEM, n=3 ( TNFα ), n=6 ( Cxcl10 , F4/80 , CD68 , CD86 , CD206 ). (G) Protein band of CD86 and GAPDH of Wild and GKKO mice liver samples (a) and their intensity profiles analyzed using ImageJ (b). Blue dotted line: Wild, red dotted line: GKKO.

    Techniques Used: Expressing, Gene Expression

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    Blocking Assay:

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    Incubation:

    Article Title: Nanoporous microstructures mediate osteogenesis by modulating the osteo-immune response of macrophages.
    Article Snippet: The osteoimmune environment plays indispensable roles in bone regeneration because the early immune environment that exists during the regenerative process promotes the recruitment and differentiation of osteoblastic lineage cells.. The response of immune cells growing on nanotopographic surfaces and the microenvironment they generate should be considered when evaluating nanotopography-mediated osteogenesis, which are topics that are generally neglected in the field.. In this study, we investigated the modulatory effects of nanoporous anodic alumina with different sized pores on macrophage responses and their subsequent effects on the osteogenic differentiation of bone marrow stromal cells (BMSCs).

    other:

    Article Title: Moderate hyperglycemia suppresses melanoma metastasis to liver
    Article Snippet: The PVDF membrane was then blocked in TBS-T (Tris-buffered saline (25 mM Tris, pH 7.4, 150 mM NaCl) containing 1 (v/v)% Tween 20) solution containing 5 (w/v)% skim milk at 25°C for 30 min. Then the PVDF membrane was probed with primary antibodies against mouse B7-2 (CD86) (1:500, sc-28347; Santa Cruz Biotechnology, Dallas, TX, USA), and GAPDH (1:1,000, sc-32233; Santa Cruz Biotechnology) in a 5% skim milk TBS-T solution at 25°C for 3 h. The membrane was further incubated with secondary antibody (anti-mouse immunoglobulin conjugated to alkaline phosphatase, Promega, Madison, WI, USA) in TBS-T at 25°C for 1 h. Finally, membranes were incubated with Western Blue Stabilized Substrate (Promega) for alkaline phosphatase at 25°C for 10 min.



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    Santa Cruz Biotechnology primary antibodies against mouse b7-2 (cd86)
    Effects of moderate hyperglycemia on the expression of TNFα , Cxcl10 , and macrophage markers in the liver. (A) TNFα expression. (B) Cxcl10 expression. (C–F) macrophage markers. Every gene expression intensity was normalized to that of GAPDH . Bars are mean ± SEM, n=3 ( TNFα ), n=6 ( Cxcl10 , F4/80 , CD68 , <t>CD86</t> , CD206 ). (G) Protein band of CD86 and GAPDH of Wild and GKKO mice liver samples (a) and their intensity profiles analyzed using ImageJ (b). Blue dotted line: Wild, red dotted line: GKKO.
    Primary Antibodies Against Mouse B7 2 (Cd86), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+mouse+b7-2+(cd86)/anti+cd86/pmc10202721-71-42-49
    Average 90 stars, based on 1 article reviews
    primary antibodies against mouse b7-2 (cd86) - by Bioz Stars, 2026-09
    90/100 stars
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    Effects of moderate hyperglycemia on the expression of TNFα , Cxcl10 , and macrophage markers in the liver. (A) TNFα expression. (B) Cxcl10 expression. (C–F) macrophage markers. Every gene expression intensity was normalized to that of GAPDH . Bars are mean ± SEM, n=3 ( TNFα ), n=6 ( Cxcl10 , F4/80 , CD68 , CD86 , CD206 ). (G) Protein band of CD86 and GAPDH of Wild and GKKO mice liver samples (a) and their intensity profiles analyzed using ImageJ (b). Blue dotted line: Wild, red dotted line: GKKO.

    Journal: Experimental Animals

    Article Title: Moderate hyperglycemia suppresses melanoma metastasis to liver

    doi: 10.1538/expanim.22-0078

    Figure Lengend Snippet: Effects of moderate hyperglycemia on the expression of TNFα , Cxcl10 , and macrophage markers in the liver. (A) TNFα expression. (B) Cxcl10 expression. (C–F) macrophage markers. Every gene expression intensity was normalized to that of GAPDH . Bars are mean ± SEM, n=3 ( TNFα ), n=6 ( Cxcl10 , F4/80 , CD68 , CD86 , CD206 ). (G) Protein band of CD86 and GAPDH of Wild and GKKO mice liver samples (a) and their intensity profiles analyzed using ImageJ (b). Blue dotted line: Wild, red dotted line: GKKO.

    Article Snippet: The PVDF membrane was then blocked in TBS-T (Tris-buffered saline (25 mM Tris, pH 7.4, 150 mM NaCl) containing 1 (v/v)% Tween 20) solution containing 5 (w/v)% skim milk at 25°C for 30 min. Then the PVDF membrane was probed with primary antibodies against mouse B7-2 (CD86) (1:500, sc-28347; Santa Cruz Biotechnology, Dallas, TX, USA), and GAPDH (1:1,000, sc-32233; Santa Cruz Biotechnology) in a 5% skim milk TBS-T solution at 25°C for 3 h. The membrane was further incubated with secondary antibody (anti-mouse immunoglobulin conjugated to alkaline phosphatase, Promega, Madison, WI, USA) in TBS-T at 25°C for 1 h. Finally, membranes were incubated with Western Blue Stabilized Substrate (Promega) for alkaline phosphatase at 25°C for 10 min.

    Techniques: Expressing, Gene Expression